Research Technician II, Duke University

  • Technician/Research Asst.
  • Utilizing Murine Models to Elucidate Cellular and Molecular Mechanisms which Regulate Bone Homeostasis

Durham, NC, USA


Description:

The Wu laboratory in the Department of Orthopaedic Surgery at Duke University seeks a motivated Research Technician (RT) to, under moderate supervision, set up and conduct experiments with appropriate controls, and continue research projects involving murine models of cancer metastasis to bone and the regulation of adipogenesis in the bone marrow. The RT will be expected to appropriately record data and report standard or variant results. The RT will also be expected to assist in general laboratory operation and inventory, and will be in charge of lab supply orders. Must be willing to collaborate with other lab personnel and trainees to accomplish goals and complete projects.


Responsibilities:

1. Under moderate supervision, conduct and evaluate experiments and assays
a. Understand and follow experimental protocols (including animal handling and procedures, tissue culture, molecular biology, immunohistochemistry, flow cytometry etc.)
b. Record, compile, interpret, and communicate results
c. Maintain an organized lab notebook
d. Participate in all training on laboratory equipment and assist with training of others (including hypoxia chamber, qPCR machine, etc.)
e. Follow University safety protocols (may include use of combustibles, radioactivity, toxic material, and/or carcinogens)
f. Perform calculations involving molarity, % solutions, and serial dilutions
2. Prepare, operate and troubleshoot equipment in experimental procedures
a. May include centrifuge, pH meter, spectrophotometer, autoclave, scintillation counter, electrophoresis unit, analytical balance, qPCR machine, gel rigs, hypoxia chamber, etc.
b. Read and follow all equipment manuals
c. Maintain and repair equipment according to manufacturer and university guidelines
3. Ensure laboratory is prepared for experiments
a. Order supplies for all laboratory personnel
b. Maintain and document lab inventory
c. Maintain sterile conditions as required
d. Prepare reagents
e. Clean equipment
4. Demonstrate an understanding of acceptable procedures and techniques
a. Recognize problems and suggest solutions
b. Assist in modifications as directed
5. Provide appropriate written documentation of experiments
a. Prepare preliminary research progress reports
b. Summarize test results appropriately
This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.


Qualifications/Preferred Skillsets:

This position requires a Bachelor's Degree in Biology/Genetics or a related field.


Link to Application (if using external Application Portal): https://career4.successfactors.com/sfcareer/jobreqcareer?jobId=145688&company=dukeuniverP1
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