Pittsburgh, PA, USA
Seeking a postdoctoral fellow to study the mechanisms whereby blood cancers resist and escape adoptive T cell immunotherapies. This newly NIH R01-funded project employs mouse leukemias created from compound oncogene knockin/knockout mice. These leukemias will be CRISPR-edited to investigate critical pathways that govern resistance and sensitivity to T cell attack. Other techniques that will be employed include single cell RNA sequencing, the creation and use of novel T cell receptor knockin mice, high parameter flow cytometry and intravital two photon microscopy. Candidates will also gain experience in writing manuscripts and grants and in giving oral presentations.
A background in immunology or cancer biology or experience in CRISPR-editing is preferred.
1. Zhou, M., Sacirbegovic, F., Zhao, K., Rosenberger, S. & Shlomchik, W. D. T cell exhaustion and a failure in antigen presentation drive resistance to the graft-versus-leukemia effect. Nature communications 11, 4227, doi:10.1038/s41467-020-17991-y (2020).
2. Morin-Zorman, S., Wysocki, C., Zhu, J., Li, H., Zorman, S., Matte-Martone, C., Kisanga, E., McNiff, J., Jain, D., Gonzalez, D., Rothstein, D. M., Lakkis, F. G., Haberman, A. & Shlomchik, W. D. In vivo dynamics of T cells and their interactions with dendritic cells in mouse cutaneous graft-versus-host disease. Blood Adv 3, 2082-2092, doi:10.1182/bloodadvances.2019000227 (2019).
3. Matte-Martone, C., Liu, J., Zhou, M., Chikina, M., Green, D. R., Harty, J. T. & Shlomchik, W. D. Differential requirements for myeloid leukemia IFN-gamma conditioning determine graft-versus-leukemia resistance and sensitivity. J Clin Invest 127, 2765-2776, doi:10.1172/JCI85736 (2017).