Researcher under the direction of Dr. Steve Grupp, assume the responsibility of the development of chimeric antigen receptor (CAR) constructs for the therapy of pediatric cancers.
The first wave of CAR T cell therapies have yielded dramatic responses in blood cancers, with wide-ranging efficacies and toxicities. There is tremendous effort under way to develop new and effective CAR T cells for other cancers including solid tumors. There is complex biology behind the construction of a tumor targeting molecules (single chain fragments, nanobodies), construction of the CAR chimeric hinge/transmembrane and endodomains, and boosting CAR function in specific tumor environments. The researcher is expected to carry out such translational immunology and synthetic biology studies, including cloning of the CAR constructs with tags for surface monitoring, in vitro killing assays and T cell activation assays, genetic manipulation of host T cells, lentiviral vector construction, RNA transfection and combinations thereof. In addition, the researcher will have opportunities for discovery as novel biology of each tumor type and CAR is revealed through iterative processing to augment the clinical impact of CAR T cell therapies.
The researcher is furthermore part of a multidisciplinary team of translational scientists, geneticists, and bioinformaticians to develop the deep understanding needed to guide therapeutic augmentation of CAR T cells. The scientist is furthermore expected to write summary reports, papers, and prepare presentations for collaborative partners.
Multicolor flow cytometry, Nanostring, and Seahorse (Agilent) knowledge/skills preferred.
-Assists faculty in the conduct of research of significant value in the basic and/or translational science area
-Plans, conducts, and manages research projects within the federal and institutional regulations and policies
-Collaborates with researchers, external agencies and institutions to develop cooperative research initiatives
-Trains technical staff, students, fellows & physicians in lab procedures
-Reviews manuscripts for peer-reviewed journals
-Assist in composing/developing applications for grants/contracts for funding of research projects
-Promotes free discussion, research progress, and overall morale
-Responsible for appropriate use of research funds
-May supervises staff and students
Required Education and Experience:
Master’s degree, 5-7 years of scientifically relevant experience required OR
Bachelor’s degree 10+ years of scientifically relevant experience required
Preferred Education, Experience:
Terminal degree (MD, PhD, DVM) preferred, 2 or more years Post-Doctoral fellowship experience; 1-3 years scientifically relevant experience
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CHOP Careers Contact
2716 South Street, 6th Floor
Philadelphia, PA 19146
Link to Application (if using external Application Portal): https://careers.chop.edu/job/Philadelphia-Research-Assoc-II-Dr_-Steve-Grupp-lab-PA-19104/800688300/?locale=en_US